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. 2013 Nov 13;28(4):414–421. doi: 10.1264/jsme2.ME13057

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Copyright © 2013 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Genetic and restriction maps of BjG30 covering genes from bll7017 to bll7033 used to construct B. japonicum USDA110 mutants. The recombinant plasmids for the deletion mutation from bll7019 to bll7021 (pK18mob-[NheI frag]::Ω) and from blr7026 to blr7029 (pK18mob-[HindIII frag]::Ω) were constructed as described in Materials and Methods. B, BsiWI; E, EcoRV; H, HindIII; N, NheI. Gene annotation (Rhizobase, http://www.kazusa.or.jp/rhizobase/): bll7017, LuxR family transcriptional regulator; bll7018, unknown protein; bll7019, AcrB/AcrD/AcrF family protein; bll7020, efflux protein; bll7021, HlyD family secretion protein; bll7022, unknown protein; blr7023 and bll7024, TetR family transcriptional regulators; bll7025, hypothetical protein; blr7026, unknown protein; blr7027, hypothetical protein; blr7028, acetoacetate decarboxylase; blr7029, β-hydroxybutyrate dehydrogenase; blr7030, unknown protein; blr7031, hypothetical protein; bll7032, putative cyclic NTP-binding protein; bll7033, LuxA-like protein.