Fig. 4.

Phylogenetic relationships based on partial 16S rRNA gene sequences recovered from salmon pink (P), chrome green (G), signal violet (V), black–gray (B), and blue–green (BG) biofilms derived from sandstone walls at Bayon by DGGE analysis. Sequences recovered in this study are shown in bold type. Numbers in parentheses are GenBank accession numbers Neighbor-joining tree: bootstrap values based on 1,000 replicates are indicated for branches supported by 50% of trees. Scale bar represents 0.02 nucleotide changes per position.

A. Phylogenetic affiliation of Cyanobacteria clade members. Bacillus subtilis DSM10^T was used as an outgroup. Dashed box indicates 16S r RNA sequences derived from rosy discoloration of European historical stone monuments (15, 20, 40).

B, Phylogenetic affiliation of Actinobacteria clade members. Escherichia coli ATCC 11775^T was used as an outgroup.

C, Phylogenetic affiliation of Chloroflexi, Proteobacteria, and Deinococcus-Thermus clade members.