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. 2014 Mar;4(1):116–127. doi: 10.1086/675641

Figure 1.

Figure 1

Localization of calcineurin (CN) in pulmonary artery endothelial cells (PAECs). A, Western blot analysis of CN in whole-cells lysates of PAECs. Whole-cell lysates of PAECs were run on 4%–12% bis-tris gels, transferred onto nitrocellulose, and subjected to Western blot analysis with antibody to pan-CN A. A ∼57-kDa band corresponding to CN A was observed. B, Immunoblot showing CN in supernatant detergent-extracted membrane fraction using octylglucoside/KI. CN A was found in the membrane fraction of PAECs. C, Immunocytochemical staining for CN in PAECs. Fluorescent images were obtained with a Leica TCS SP2 confocal laser scanning microscope fitted with a 63× water immersion objective. Arrows highlight membrane localization of positive CN staining. D, Enlarged detail of cell-cell border staining in C.