Figure 3.

Calcineurin inhibitory peptide (CIP) reduces thapsigargin (TG)-induced calcineurin (CN) activity in pulmonary artery endothelial cells (PAECs). PAECs were transiently transfected with a nuclear factor of activated T cells (NFAT)–responsive luciferase reporter plasmid, an indicator of CN phosphatase activity. CN activity is shown as relative light units (RLU) of luciferase normalized to total protein. CN activity increased upon TG treatment, and this increase was attenuated in CIP-treated cells. Each bar represents the mean + SEM of at least 3 independent experiments. Asterisk indicates significant differences from TG-treated cells.