Figure 5.

Calcineurin inhibitory peptide (CIP) increases phosphorylation of stromal interaction molecule 1 (STIM1) in pulmonary artery endothelial cells (PAECs). A, Western blot analysis of CIP-treated cells. Supernatant octylglucoside/KI-extracted membrane fractions of cells treated with or without 5-minute thapsigargin (TG) treatment and with or without CIP and subjected to Western blot analysis with antiphosphoserine antibody. A phosphospecific band at ∼85 kDa was observed that increased in intensity with TG and/or CIP treatment. B, Western blot densitometry results from A. ImageJ software was used to measure the densitometry of bands. Data are represented as percent change in phosphorylation, based on band density of TG-treated cells or untreated cells. Each bar represents the mean ± SEM of 2 independent membrane preparations. Asterisk indicates significant difference from non-CIP-treated controls.