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Calcineurin inhibitory peptide (CIP) reduces thapsigargin-induced intercellular gap formation in pulmonary artery endothelial cells (PAECs). A, PAECs grown on glass coverslips exhibit confluent monolayer at time 0. B, Thapsigargin treatment induces intercellular gap formation, as denoted by arrows. C, PAECs exhibit confluent monolayer after 1-hour pretreatment with 50 μM CIP. D, CIP-pretreated cells do not form intercellular gaps after thapsigargin treatment.
