Figure 4.

Hydrocortisone (HC) does not affect expression of Nox1 and Nox4 in fetal pulmonary artery smooth muscle cells (FPASMCs) from lambs with persistent pulmonary hypertension of the newborn (PPHN). PPHN FPASMC were exposed to 21% O₂–5% CO₂ or 95% O₂–5% CO₂ ± 100 nM HC for 24 hours. Cells were harvested for total protein and subjected to Nox1 (A) and Nox4 (B) Western blot analysis, with β-actin normalization. Data are shown as means ± SEM (Nox1: n = 11 for 21% untreated, 95% untreated, 95% plus 100 nM HC, n = 10 for 21% plus 100 nM HC; Nox4: n = 9 for 21% untreated and 95% untreated, n = 8 for 21% plus 100 nM HC, 95% plus 100 nM HC).