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. 2013 Sep;3(3):675–691. doi: 10.1086/674440

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© 2013 by the Pulmonary Vascular Research Institute. All rights reserved.

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Asymmetric dimethylarginine (ADMA) and dimethylarginine dimethylaminohydrolase I (DDAHI) selectively regulate connexin (Cx) 43 expression. A, B, Effects of ADMA and DDAHI on Cx43 protein expression and phosphorylation in human pulmonary artery endothelial cells (HPAECs). C, Corresponding representative Western blots showing expression of Cx37, Cx40, and Cx43. D, Concentration-dependent reduction of Cx43 expression in HPAECs. E, F, ADMA reduces the levels of cytoskeleton-bound Cx43, and DDAHI prevents this effect. G, Intracellular distribution of Cx43 and vascular endothelial–cadherin in HPAECs treated as indicated. Confocal microscopy. Scale bar = 10 μm. H, Expression levels of DDAHI in cells 24 hours postinfection with adenoviruses for overexpression of DDAHI. One asterisk: , comparison with controls; Delta: ; two Deltas: , comparison with ADMA. . VE: vascular endothelial; AdGFP: adenoviruses for overexpression of green fluorescent protein.

Inline graphic — Asymmetric dimethylarginine (ADMA) and dimethylarginine dimethylaminohydrolase I (DDAHI) selectively regulate connexin (Cx) 43 expression. A, B, Effects of ADMA and DDAHI on Cx43 protein expression and phosphorylation in human pulmonary artery endothelial cells (HPAECs). C, Corresponding representative Western blots showing expression of Cx37, Cx40, and Cx43. D, Concentration-dependent reduction of Cx43 expression in HPAECs. E, F, ADMA reduces the levels of cytoskeleton-bound Cx43, and DDAHI prevents this effect. G, Intracellular distribution of Cx43 and vascular endothelial–cadherin in HPAECs treated as indicated. Confocal microscopy. Scale bar = 10 μm. H, Expression levels of DDAHI in cells 24 hours postinfection with adenoviruses for overexpression of DDAHI. One asterisk: , comparison with controls; Delta: ; two Deltas: , comparison with ADMA. . VE: vascular endothelial; AdGFP: adenoviruses for overexpression of green fluorescent protein.