Figure 11.

Probenecid rescues the intracellular trapping of the KDF mutant of bone morphogenetic protein receptor type 2 (BMPR2). EA.hy926 cells were transfected with Flag-tagged wild-type (wt) or the KDF species of BMPR2 and then, beginning 5 hours later, exposed to either probenecid (PB; 2 mM) or 4-phenylbutyrate (4PBA; 5 mM) or left untreated. One day later, the subcellular localization of the respective BMPR2 species was evaluated using anti-Flag antibody. A illustrates some of the representative imaging data (scale bar = 10 μm). B presents histograms of the phenotypic distribution of BMPR2 species in single cells (n = number of cells enumerated) under each experimental condition using the method described in the legend for Figure 6. Asterisks indicate indicates for the KDF distributions in treated cultures using a multigroup analysis of variance in comparison with that derived from the untreated culture.