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. 2013 Sep;3(3):622–631. doi: 10.1086/674309

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© 2013 by the Pulmonary Vascular Research Institute. All rights reserved.

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Analysis of lumen morphology as a function of gel collagen concentration and oxidized low-density lipoprotein (oxLDL). A, Inner area, which represents the area of the capillary lumen (black diamonds), and the outer area (gray diamonds), which represents the lumen and endothelial cells, or total area of the capillary. The inner area increases significantly with increasing gel collagen content (), and the outer area shows a similar trend (). The difference between the inner and outer areas represents cell wall thickness, or endothelial cell area, which shows a trend to increase with the gel collagen concentration. B, Inner (black squares) and outer (gray squares) areas in cells treated with oxLDL demonstrated the same trend but a reduced difference between inner and outer areas corresponding to a decrease in cell wall thickness. C, Overlapping the areas of the lumens of control (black diamonds) and oxLDL-treated (black squares) cells demonstrates that oxLDL-treated cells form larger lumens than do control cells in most stiffnesses () except for the very soft and the very stiff gels. D, Overlapping the outer areas of control (gray diamonds) and oxLDL-treated (gray squares) cells demonstrates that oxLDL has no effect on this parameter.

Inline graphic — Analysis of lumen morphology as a function of gel collagen concentration and oxidized low-density lipoprotein (oxLDL). A, Inner area, which represents the area of the capillary lumen (black diamonds), and the outer area (gray diamonds), which represents the lumen and endothelial cells, or total area of the capillary. The inner area increases significantly with increasing gel collagen content (), and the outer area shows a similar trend (). The difference between the inner and outer areas represents cell wall thickness, or endothelial cell area, which shows a trend to increase with the gel collagen concentration. B, Inner (black squares) and outer (gray squares) areas in cells treated with oxLDL demonstrated the same trend but a reduced difference between inner and outer areas corresponding to a decrease in cell wall thickness. C, Overlapping the areas of the lumens of control (black diamonds) and oxLDL-treated (black squares) cells demonstrates that oxLDL-treated cells form larger lumens than do control cells in most stiffnesses () except for the very soft and the very stiff gels. D, Overlapping the outer areas of control (gray diamonds) and oxLDL-treated (gray squares) cells demonstrates that oxLDL has no effect on this parameter.