Table 3.
Top five networks in sham-operated C57BL/6 mice that were treated with GW0742
| Associated network functions | Molecules in network | Focus molecules | Score |
|---|---|---|---|
| Cell cycle, cellular development, cellular growth and proliferation | BMP4, CCNA1, CCNE1, CCNE2, CDC25A, CDK2, CDKN1A, CDX2, CHEK1, CSF1 (includes EG:12977), CUL1, cyclin A, cyclin E, ELAVL1, FOXM1, HDAC3, HDAC4, histone H1, KLF4, LGALS1, MAP2K1, NCOR1, NCOR2, NPM1, OSM, PCNA, POLR2A, PRKCE, PYHIN1, RBL2 (includes EG:100331892), SFN, SKP2 (includes EG:27401), SP3, TOPBP1, TSPYL2 | 2 | 3 |
| Amino acid metabolism, small-molecule biochemistry, connective tissue disorders | PPAT, SREBF1 | 1 | 3 |
| Amino acid metabolism, auditory and vestibular system development and function, cellular development | DIO2, MARCH6 | 1 | 3 |
| Cell death, protein synthesis, cellular development | DGAT2, ERN1 | 1 | 3 |
| Developmental disorder, genetic disorder, metabolic disease | CTSD, HEXB, MREG | 1 | 3 |
Animals underwent a sham operation and after several days were orally dosed with vehicle or GW0742 (30 mg/kg). After 14 days, the right ventricle was excised, and RNA was extracted from each sample. Gene expression was measured using the Illumina MouseRef-8v3 BeadChip array, and data were analyzed using the syntax package R (ver. 2.3.1) with Bioconductor and limma. Genes that were significantly modified, as calculated using a moderated t test followed by Benjamini-Hochberg false-discovery rate correction, were imported into Ingenuity Pathway Analysis software. The table shows the top five networks identified from genes differentially expressed in C57BL/6 mice treated with GW0742 compared with sham-operated controls.