Table 5.

Top five networks in C57BL/6 mice undergoing pulmonary artery banding (PAB) that were treated with 30 mg/kg GW0742

Associated network functions	Molecules in network	Focus molecules	Score
Cellular development, embryonic development, cell death	ACADS, ADAM12, ALDOA, ANKRD40, APP, ARHGEF2, CFL2, CKAP2, CRTC1, CSRP3, DYRK1A, ENSA, EPN2, HGF, HSPH1, IGF1R, IL5, KIF5C, LBR (includes EG:368360), LIF, LMO7, MAP4, MAPK1, NDUFV3, NUMBL, PACSIN3, PLOD1, PLXDC1, PSMB5, RAE1, RGS12, SH3BP4, TLR3, TP53 (includes EG:22059), YWHAG	13	27
Cell death, cellular movement, cell morphology	ADAM10, APOA1, AQP1, AQP7, CACNA2D1, calcineurin protein(s), calpain, DNAI1, DNAJB6, ERK, FCER1G, FHOD1, FOS, GATA6, HBB, HOXA10, HTT, JUP, KLF1, LCN2, LPAR1, MAP7D1, MDH2 (includes EG:17448), PAK1, PCYT1A, PDGFB, PECAM1, PRKCB, REM1, RGS5, SIRPA, SUZ12, TGFA, TGFB1 (includes EG:21803), VASP	12	25

^NoteAnimals’ pulmonary arteries were occluded, and after several days animals were orally dosed with vehicle or 30 mg/kg GW0742. After 14 days, the right ventricle was excised, and RNA was extracted from each sample. Gene expression was measured using the Illumina MouseRef-8v3 BeadChip array, and data were analyzed using the syntax package R (ver. 2.3.1) with Bioconductor and limma. Genes that were significantly modified, as calculated using a moderated t test followed by Benjamini-Hochberg false discovery rate correction, were imported into Ingenuity Pathway Analysis software. The table shows the top five networks identified from genes significantly expressed in PAB C57BL/6 mice treated with GW0742 compared with vehicle controls.