Table 1.
Semiquantitative analysis of TZG-induced PGIS and CD45 immunoreactivity
|
COX-2flox/flox
|
Tie2Cre COX-2flox/flox
|
Control
|
||
|---|---|---|---|---|
| TZG | TZG plus NS-398 | TZG | Saline | |
| A. PGIS and CD45 striatal immunoreactivity 12 hours after TZG or saline injection | ||||
| PGIS | ++ | − | − | − |
| CD45 | +++ | +++ | +++ | − |
|
| ||||
| 4 hours | 8 hours | 12 hours | 24 hours | |
|
| ||||
| B. Time course of PGIS striatal immunoreactivity in wild type mice after TZG injection | ||||
| PGIS | − | + | ++ | ++ |
Notes: +++, high density of cellular signals; ++, moderate density; +, low density; −, no signal.
Abbreviations: PGIS, prostacyclin synthase; TZG, (RS)-(tetrazol-5-yl)glycine.