Figure 10.

EGCG inhibits CpG-ODN-induced migration of DU145 and PC3 cells.

Notes: Cell migration assays were performed using a modified Boyden chamber containing fibronectin-coated polycarbonate membrane filter (8 μm pore size). DU145 or Pc3 cells (2×10⁵) were pretreated or not with EGCG (40 μg/ml) for 24 hours followed by transfection with CpG-ODN (1 μM). Cells were plated in the upper chamber and the lower chamber contained culture medium with 10% FBs. Cells were incubated for 24 hours at 37°C in 5% CO₂. Nonmigrated cells were scraped from the upper surface of the membrane with a cotton swab, and migrated cells remaining on the bottom surface were trypsinized and counted with a hemocytometer. Data shown are means ± SD of experiments performed in triplicate. Student’s t-test: *P<0.001.

Abbreviations: CpG-ODN, CpG oligodeoxynucleotides; EGCG, epigallocatechin-3-gallate; FBS, fetal bovine serum; SD, standard deviation.