Figure 6.

EGCG inhibits CpG-ODN-induced NF-κB transcriptional activation.

Notes: (A) DU145 and (B) PC3 cells were cotransfected with NF-κB-luciferase plasmid (1.0 μg) and Renilla luciferase plasmid (0.1 μg). After 24 hours, cells were transfected with CpG-ODN (1 μM) with or without EGCG pretreatment (40 μg/ml) for 24 hours. NF-κB luciferase activity was normalized to Renilla luciferase activity and represented as fold induction. Data shown are means ± SD of experiments performed in triplicate. Student’s t-test: values as indicated.

Abbreviations: CpG-ODN, CpG oligodeoxynucleotides; EGCG, epigallocatechin-3-gallate; RLU, relative luciferase units; SD, standard deviation; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells.