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. 2014 Jun 17;7:89–101. doi: 10.2147/JIR.S61365

Figure 7.

Figure 7

EGCG inhibits CpG-ODN-induced nuclear translocation of NF-κB.

Notes: DU145 cells were grown on glass cover slips and mock treated or transfected with CpG-ODN (1 μM) for 1 hour with or without EGCG pretreatment (40 μg/mL) for 24 hours. Cells were incubated with goat anti-rabbit polyclonal NF-κB p65 subunit antibody (green) for 1 hour followed by alexa Fluor 488 donkey anti-rabbit secondary antibody and mounted in VECTASHIELD® (Vector Labs, Burlingame, CA, USA) with DAPI to stain nuclei (blue). Images were merged to determine nuclear localization. Representative images of cells visualized under confocal microscope at 60× are shown.

Abbreviations: CpG-ODN, CpG oligodeoxynucleotides; DAPI, 4′,6-diamidino-2-phenylindole; EGCG, epigallocatechin-3-gallate; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; TNF, tumor necrosis factor.