Figure 8.

EGCG sequesters NF-κB p65 subunit in the cytosol.

Notes: DU145 cells were transfected with CpG-ODN (1 μM) for 1 hour with or without EGCG pretreatment (40 μg/ml) for 24 hours. Levels of NF-κB p65 subunit in the (A) nuclear extract normalized to histone H2A or (B) cytosol normalized to β-actin levels was determined by immunoblot analysis and compared to levels of inhibitory subunit IκBα.

Abbreviations: CpG-ODN, CpG oligodeoxynucleotides; EGCG, epigallocatechin-3-gallate; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells.