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. 2014 Jun 17;7:89–101. doi: 10.2147/JIR.S61365

Figure 9.

Figure 9

EGCG inhibits CpG-ODN-induced activity of MMP-9 and -2.

Notes: DU145, Pc3, and Lncap cells were transfected with CpG-ODN (1 μM) for 8 hours with or without EGCG pretreatment (40 μg/ml) for 24 hours. Activity of MMP-9 and -2 in culture supernatants was determined by electrophoresis in 8% SDS-Page gels containing 0.1% gelatin. Gels were stained and clear areas representing MMP activity were imaged. Results are representative of three independent experiments.

Abbreviations: CpG-ODN, CpG oligodeoxynucleotides; EGCG, epigallocatechin-3-gallate; MMP, matrix metalloproteinase; SDS-Page, sodium dodecyl sulfate polyacrylamide gel electrophoresis.