FIG. 4.

Mean serum LH levels in female WT and Kiss1r KO mice exposed to a positive feedback regimen of constant elevated E₂ after several days of GnRH priming. A) LH levels in mice 15 min after the final GnRH priming injection. GnRH stimulated robust LH secretion in both genotypes, indicating a responsive pituitary. *Significantly different from non-GnRH treated group (P < 0.01). B) Two days after the last GnRH priming treatment, mice were OVX and given E₂, and blood was collected 2 days later at a morning (1000 h) or an evening (lights off; 1800 h) time point. **Significantly different from all other groups. All data are expressed as the mean ± SEM for each group.