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Representative photomicrographs of cfos mRNA coexpression in GnRH neurons in WT and Kiss1r KO female mice exposed to an LH surge paradigm of constant elevated E2 and sacrificed in the evening. Yellow arrows denote examples of GnRH cells (red fluorescence) with significant cfos (silver grains) coexpression. Blue arrows designate example GnRH neurons that did not have cfos induction.
