FIG. 6.

Mean percent of GnRH neurons coexpressing cfos in female mice exposed to an LH surge paradigm of constant elevated E₂. Two days after E₂ implantation, brains were collected from mice at either a morning (1000 h) or an evening (1800 h) time point. Brains were assayed for cfos induction in GnRH cells using double-label ISH. Mean levels of cfos-GnRH coexpression in mice from different time-points were quantified in three brain regions within the GnRH neural system as well as the entire GnRH population. *Significantly different from all other groups (P < 0.01). All data are expressed as the mean ± SEM for each group.