Figure 5. Cathepsin S and PAR2 peptide agonists induce PKC Ser660 phosphorylation in HeLa cells.

The Western blot was performed on HeLa cells that had been transfected with PAR2 cDNA. Treatments included SLIGRL (lane 1), SLIGKVDGTS (lane 2), KVDGTS (lane 3) and cathepsin S (lane 4). Non-transfected HeLa cells treated with SLIGRL (10 µM) (lane 5) and PAR2-transfected but untreated HeLa cells (lane 6) served as controls. SLIGRL (10 µM), SLIGKVDGTS (10 µM), KVDGTS (100 µM) and cathepsin S (1 µM).