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. 2014 Jun 25;9(6):e99702. doi: 10.1371/journal.pone.0099702

Figure 6. Cathepsin S cleavage of the PAR2 N-terminus requires glycine40.

Figure 6

a) Luciferase luminescence was measured after cathepsin S (2 µM) treatment of HeLa cells expressing luciferase-PAR2 (bar 1), PAR2M1 (bar 2), PAR2M2 (bar 3), and PAR2M3 (bar 4). Luminescence of the transfected cells without cathepsin S treatment was subtracted from each of the measurements. b) Western blots were performed on supernatants of luciferase-PAR2 and PAR2 mutant-transfected HeLa cells following treatment with cathepsin S. Luciferase-PAR2, cathepsin S(−) (lane 1); luciferase-PAR2, cathepsin S (+) (lane 2); PAR2M1, cathepsin S (−) (Lane 3); PAR2M1 cathepsin S (+) (lane 4); PAR2M2, cathepsin S (−) (lane 5); PAR2M2, cathepsin S (+) (lane 6); PAR2M3, cathepsin S (−) (lane 7); PAR2M3, cathepsin S (+) (lane 8) and non-transfected HeLa cells, cathepsin S (+) (lane 9). Molecular weight markers on the left side of the blot are in kDa.