Figure 7. Cathepsin S fails to activate mutant PAR2 but KVDGTS retains activity on mutant PAR2.

a) Calcium imaging was performed in HeLa cells transfected with native or mutant PAR2 receptors following treatment with cathepsin S. Cathepsin S (2 µM) was able to stimulate native PAR2 and PAR2M1 but not PAR2M2 or PAR2M3. b) In contrast, KVDGTS activated PAR2 and all three substitution mutants. Cathepsin S (2 µM), KVDGTS (100 µM).