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. 2014 Jun 25;9(6):e99702. doi: 10.1371/journal.pone.0099702

Table 1. LC/MS/MS data from cathepsin S digestion of the human PAR2 N-terminal peptide G28TNRSSKGRSLIGKVDGTSHVTGKGVT.

P1 position Sequence Counts
V D43GTSHVTGKGVT 4
I G40KVDGTSHVTGKGVT 3
- G28TNRSSKGRSL 163
- G28TNRSSKGRSLI 9
- G28TNRSSKGRSLIGK 4
- G28TNRSSKGRSLIGKVD 54
- G28TNRSSKGRSLIGKVDGTSHVT 38
- G28TNRSSKGRSLIGKVDGTSHVTG 2
- G28TNRSSKGRSLIGKVDGTSHVTGKGVT 343
D G44TSHVTGKGVT 8
L I39GKVDGTSHVT 7
L I39GKVDGTSHVTG 7
L I39GKVDGTSHVTGKGVT 24
G K41VDGTSHVT 13
G K41VDGTSHVTG 8
G K41VDGTSHVTGK 5
G K41VDGTSHVTGKG 1
G K41VDGTSHVTGKGV 5
G K41VDGTSHVTGLGVT 29
G R36SLIGKVD 4
S S33KGRSLIG 4
R S32SKGRSLIG 5
G T29NRSSKGRSLIG 4
G T45SHVTGKGVT 3
K V42DGTSHVT 1
K V42DGTSHVTG 2
K V42DGTSHVTGKGVT 4

MS/MS analysis of cathepsin S cleavage of synthetic N-terminal PAR2. Column 1 lists the amino acid in the P1 postion. Column 2 lists the identified cleaved peptide. Column 3 lists the number of times the cleaved peptide occurred in the scan. Details of the method are provided in the Experimental Procedures section.