Table 1. LC/MS/MS data from cathepsin S digestion of the human PAR2 N-terminal peptide G28TNRSSKGRSLIGKVDGTSHVTGKGVT.
P1 position | Sequence | Counts |
V | D43GTSHVTGKGVT | 4 |
I | G40KVDGTSHVTGKGVT | 3 |
- | G28TNRSSKGRSL | 163 |
- | G28TNRSSKGRSLI | 9 |
- | G28TNRSSKGRSLIGK | 4 |
- | G28TNRSSKGRSLIGKVD | 54 |
- | G28TNRSSKGRSLIGKVDGTSHVT | 38 |
- | G28TNRSSKGRSLIGKVDGTSHVTG | 2 |
- | G28TNRSSKGRSLIGKVDGTSHVTGKGVT | 343 |
D | G44TSHVTGKGVT | 8 |
L | I39GKVDGTSHVT | 7 |
L | I39GKVDGTSHVTG | 7 |
L | I39GKVDGTSHVTGKGVT | 24 |
G | K41VDGTSHVT | 13 |
G | K41VDGTSHVTG | 8 |
G | K41VDGTSHVTGK | 5 |
G | K41VDGTSHVTGKG | 1 |
G | K41VDGTSHVTGKGV | 5 |
G | K41VDGTSHVTGLGVT | 29 |
G | R36SLIGKVD | 4 |
S | S33KGRSLIG | 4 |
R | S32SKGRSLIG | 5 |
G | T29NRSSKGRSLIG | 4 |
G | T45SHVTGKGVT | 3 |
K | V42DGTSHVT | 1 |
K | V42DGTSHVTG | 2 |
K | V42DGTSHVTGKGVT | 4 |
MS/MS analysis of cathepsin S cleavage of synthetic N-terminal PAR2. Column 1 lists the amino acid in the P1 postion. Column 2 lists the identified cleaved peptide. Column 3 lists the number of times the cleaved peptide occurred in the scan. Details of the method are provided in the Experimental Procedures section.