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. 2014 Jun 25;9(6):e99741. doi: 10.1371/journal.pone.0099741

Figure 6. Down regulation of FtsHi4 by RNAi leads to defects in chloroplast development.

Figure 6

A, 2-week-old seedlings of WT grown on half MS medium. B, 2-week-old seedlings of RNAi-FtsHi4 line grown on half MS medium. C, 5-week-old plants of WT grown in soil. D, 5-week-old plants of RNAi-FtsHi4 line grown in soil. E, Chloroplasts from a 5-week-old leaf of wild-type plant. These chloroplasts are well developed, with redundant grana interconnected by stroma thylakoids. Enlargement of such a chloroplast is shown in (G). F, Plastids from 5-week-old leaf of RNAi-FtsHi4 plants, these plastids had straight thylakoids but lacked granal lamellae. Enlargement of such a plastid is shown in (H). ItoJ, qRT-PCR and immunoblot analysis showing expression levels of FtsHi4 in leaves excised from 2-week seedlings described in A and B. Chloroplast proteins were further detected in 2-week seedlings of the RNAi mutant using a specific antibody.