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Cells were incubated with different concentrations of CAPE for 4(A) total protein or (B) protein fractions. The amount of phosphorylated FoxO4 (pSer194) in total protein and the amount of total FoxO4 in the nuclear fraction were determined. One representative out of three is shown, data (mean ± SD) are given as fold increase of FoxO4 protein amount compared to the vehicle control.
