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. 2014 Jun 25;9(6):e100594. doi: 10.1371/journal.pone.0100594

Figure 3. Hepatocyte growth factor increases mRNA expression of myogenic factors and myostatin.

Figure 3

Total RNA was purified from the same samples as used in figure 2. RT-qPCR analysis of mRNA expression levels in tibialis anterior of shows hepatocyte growth factor increases myogenic factors MyoD, myogenin (A,B). Muscle negative regulation and protein breakdown pathway myostatin, MAFbx and MuRF1 (C-E) were increased following a single injection with hepatocyte growth factor. RU = Relative Units. N = 3 for all time points, except for 60 minH ( = a 10 times higher dose of hepatocyte growth factor [200 ng HGF/g body weight] sacrificed after 60 min) where N = 2. Error bars are SD; One-way ANOVA with Bonferroni post hoc correction for multiple comparisons was used to assess differences among groups for each dependent variable. *denotes significant (P<0.05). Data are representative of four independent experiments.