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. 2014 Jun 25;9(6):e100594. doi: 10.1371/journal.pone.0100594

Figure 6. Hepatocyte growth factor and leukemia inhibitory factor treatment increases the mitosis of satellite cells in vivo during hypoxia.

Figure 6

Tibialis anterior sections were incubated with antibodies specific for satellite cell marker Pax7, proliferation marker Ki67 and DNA synthesis marker BrdU. Treatment of hypoxic mice with hepatocyte growth factor alone or in combination with leukemia inhibitory factor increased ratio of satellite cells undergoing divisions in following one HGF injection (N = 11, pink) compared to PBS (N = 12, blue) and multiple HGF/LIF (N = 13, red) injections compared to multiple PBS (N = 14, dark blue) or (A,C). Treatment of hypoxic mice with hepatocyte growth factor in combination with leukemia inhibitory factor (N = 13, red) increased number of cells with incorporated BrdU following DNA synthesis in following compared to multiple injections with PBS (N = 14, dark blue) (B,D). Bar in picture is 50 µm. Error bars are SD; Statistical significance was determined by a two-tailed Student's t–test. *denotes significant (P<0.05). Data are representative of one experiment.