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. 2013 Sep 4;28(3):346–353. doi: 10.1264/jsme2.ME13042

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Copyright © 2013 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Phylogenetic analysis showed the affiliation of isolates in this study. The phylogenetic tree was based on 16S rRNA gene sequences of selected Nitrospira. The tree was constructed using the neighbor-joining algorithm. Numbers at branch nodes are bootstrap values. Previously defined sublineages (6) of the genus Nitrospira (Roman numbers) are shown. Clones obtained previously from soils and activated sludge are classified into separate clusters. Scale bar corresponds to 1% estimated sequence divergence.