Table 2. Comparison of COG distribution in the virulence genes with the B. melitensis 16 M genome COG distribution.

COG	B. melitensis genome	Virulence genes	Comparison
	Frequency(%)	Frequency(%)	P value
A	1 (0.03)	0 (0)	1
B	1 (0.03)	0 (0)	1
C	172 (5.1)	10 (6.25)	0.51
D	27 (0.80)	0 (0)	0.63
E	326 (9.65)	9.5 (5.94)	0.15
F	74 (2.2)	10.5 (6.56)	0.001*
G	187 (5.53)	11.5 (7.19)	0.29
H	133 (3.93)	3.5 (2.19)	0.36
I	114 (3.37)	1 (0.63)	0.06
J	165 (4.88)	3.3 (2.06)	0.08
K	190 (5.62)	16.7 (10.44)	0.008*
L	107 (3.17)	5.5 (3.44)	0.68
M	169 (5.0)	17 (10.62)	0.002*
N	31 (0.92)	7.5 (4.69)	<0.001*
NC	573 (16.95)	11 (6.87)	<0.001*
O	121 (3.58)	8.5 (5.31)	0.18
P	157 (4.65)	3 (1.88)	0.09
Q	63 (1.86)	1 (0.62)	0.37
R	318 (9.41)	9 (5.62)	0.10
S	268 (7.94)	10 (6.25)	0.63
T	92 (2.72)	7 (4.38)	0.45
U	51 (1.50)	12.5 (7.81)	<0.001*
V	38 (1.13)	2 (1.25)	0.70
W	1 (0.03)	0 (0)	1
Total	3379 (100)	160 (100)

P value obtained with chi-squared test and Fisher exact test,

*p-value ≤0.05. Abbreviations: COG: cluster of orthologous groups, A: RNA processing and modification, B: chromatin structure and dynamics, C: energy production and conversion, D: cell cycle control, cell division, chromosome partitioning, E: amino acid transport and metabolism, F: nucleotide transport and metabolism, G: carbohydrate transport and metabolism, H: coenzyme transport and metabolism, I: lipid transport and metabolism, J: translation, ribosomal structure and biogenesis, K: transcription, L: replication, recombination and repair, M: cell wall/membrane/envelope biogenesis, N: cell motility, NC: not in COG, O: posttranslational modifications, protein turnover, chaperones, P: inorganic ion transport and metabolism, Q: secondary metabolites biosynthesis, transport and catabolism, R: general function prediction only, S: function unknown, T: signal transduction mechanisms, U: intracellular trafficking, secretion and vesicular transport, V: defense mechanisms, W: extracellular structures.