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. 2014 Jun 19;14:456. doi: 10.1186/1471-2407-14-456

Figure 1.

Figure 1

Single cell isolation and identification. Individual tumor cells (TCs) from primary or metastatic tissue, blood, or bone marrow were isolated by the MagSweeper and immunostained (200×). Panel (A) shows examples of CTCs and DTCs (CK+, CD45-, DAPI+); small round circles in two frames of (A) are autofluorescing residual magnetic microbeads; Panel (B) shows an example of TCs from tumor tissue (CK+, CD45-, DAPI+); Panel (C) shows examples of WBCs (CK-/very weak, CD45+, DAPI+). Green = cytokeratin; red = CD45; blue = DAPI nuclear stain.