Figure 1.

In A, LCM retrieval of endothelial cells from paraffin embedded tissue sections (a - before dissection, b - removal of blood cells, c- capture of endothelial cells). B, RNA purity control, using VEGFR2, a marker for endothelial cells, and E-cadherin, a marker for cells of ectodermal origin. C, D, E, real-time PCR to quantify Grp78, ATF4 and CHOP expression in TEC compared to NEC and primary endothelial cells. Specimens were obtained from human oral squamous cell carcinoma biopsies. Data presented from real-time PCR experiments reflect the expression levels of Grp78, ATF4 and CHOP normalized to β-tubulin. Values are means ± SD. Columns, means of individual experiments; Bars, SD. ***p<0.001.