Table 3. Vertical transmission of Fusarium mycoviruses.
Conidial generation | Vertical transmission rate (%) | |||
FgV1 | FgV2 | FgV3 | FgV4 | |
1st | 93.3 [28/30] | 10.0 [3/30] | 56.7 [17/30] | 100 [30/30] |
2nd | 93.3 [28/30] | 83.3 [25/30] | 46.7 [14/30] | 40.0 [12/30] |
3rd | 100 [30/30] | 100 [30/30] | 76.7 [23/30] | 36.7 [11/30] |
Vertical transmission was measured as the percentage of FgVs-positive isolates among the total number of single-conidium isolates. The presence of viral dsRNA was determined by RT-PCR analysis. The mycelial plugs obtained from virus-positive isolates were inoculated into CMC liquid medium for the next conidial generation. Numbers in squared brackets indicate the number of virus-positive isolates/total number of single-conidium isolates.