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. 2014 Jun 25;9(6):e100989. doi: 10.1371/journal.pone.0100989

Table 3. Vertical transmission of Fusarium mycoviruses.

Conidial generation Vertical transmission rate (%)
FgV1 FgV2 FgV3 FgV4
1st 93.3 [28/30] 10.0 [3/30] 56.7 [17/30] 100 [30/30]
2nd 93.3 [28/30] 83.3 [25/30] 46.7 [14/30] 40.0 [12/30]
3rd 100 [30/30] 100 [30/30] 76.7 [23/30] 36.7 [11/30]

Vertical transmission was measured as the percentage of FgVs-positive isolates among the total number of single-conidium isolates. The presence of viral dsRNA was determined by RT-PCR analysis. The mycelial plugs obtained from virus-positive isolates were inoculated into CMC liquid medium for the next conidial generation. Numbers in squared brackets indicate the number of virus-positive isolates/total number of single-conidium isolates.