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Expression of P2X4R following UUO: (A) Immunohistochemistry for P2X4R in WT mice following UUO: There was a linear positive staining on the luminal surface of tubular epithelial cells in non-operated kidneys, whereas the positive staining was internalized after 7 and 14 days in UUO-operated kidneys. Original magnification ×70. (B) Increased expression of P2X4R mRNA following UUO detected by RT–qPCR.
