Figure 6. lin-28^dF30 Mutant Germaria Suggest Abnormal GSC Differentiation and Early Egg Chamber Fusion.

(A–B’) Rounded Spectrin marks the GSCs (asterisk), while it stains branched fusomes as the cells differentiate towards the posterior end (arrow) in lin-28^dF30/+ germaria (n = 27). In lin-28^dF30 germaria, misdifferentiated GSCs retained a round fusome (asterisks) (n = 23). (C–D’) Orb expression in the germaria started in R1 (arrow) and was reinforced at stage 2 and 3, when the cyst was encapsulated by follicle cells to form the egg chamber. In lin-28^dF30 germaria, more cystoblasts were expressing Orb in R1 (arrow) (76.9%; n = 39). Scale bars represent 25µm.