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BrdU-positive nuclei co-localized with ChAT. Confocal z-stack image from representative tissue section taken 12 weeks after METH. BrdU-positive nuclei are labeled in green with FITC (A). ChAT-positive interneurons are labeled with Cy3 in red (B). Co-labeled cells can be seen in the same tissue section in panel C. Arrows indicate co-labeled interneurons. Few BrdU-positive cells co-labeled with ChAT can be seen at 8 and 12 weeks post-METH (D). *p<0.001, **p<0.01 (n=4).
