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. Author manuscript; available in PMC: 2014 Jun 25.
Published in final edited form as: J Alzheimers Dis. 2014 Jan 1;41(2):499–513. doi: 10.3233/JAD-132792

Fig. 8.

Fig. 8

Propofol attenuates Aβ-induced caspease-3 activation through GABA receptors in H4 naïve cells. A) Five μM Aβ (lanes 5-6) induces caspase-3 activation as compared to saline (lanes 1-2). 100 μM propofol (lanes 7-8) attenuates the caspase-3 activation induced by 5 μM Aβ (lanes 5-6) in H4 naïve cells. 20 μM flumazenil (lanes 9-10) inhibits the attenuation effect of 100 μM propofol (lanes 7-8) on the Aβ-induced caspase-3 activation (lanes 5-6). 20 μM flumazenil alone (lanes 3-4) has no significant effects on caspase-3 activation as compared to saline (lanes 1-2) in H4 naïve cells. There is no significant difference in amounts of β-Actin among all the groups. B) Quantification of the western blot shows that 5 μM Aβ (grey bar) induces caspase-3 activation as compared to saline (white bar), 100 μM propofol (net bar) attenuates the Aβ-induced caspase-3 activation (grey bar) in H4 naïve cells. Twenty μM flumazenil (dot bar) inhibits the attenuation effect of 100 μM propofol (net bar) on the Aβ-induced caspase-3 activation (grey bar). There is no difference on caspase-3 activation between the treatment of saline (white bar) and 20 μM flumazenil alone (black bar) in H4 naïve cells. FL, full length, Aβ, amyloid-β protein. n = 6.