Figure 1. TLR ligands differentially affected the number of memory CD8⁺ T cells that form following DC vaccination.

B6 mice containing a small number (~5×10³/mouse) of naive P14 CD8⁺ T cells were immunized with either DC-33 i.v. alone or in combination with various TLR agonists i.p. as indicated (see methods for doses), an additional group of mice were served as untreated controls. (A) Spleens were harvested on day 7 and 45 days post immunization and the antigen-specific P14 CD8⁺ T cells plotted in the dot plots. (B) Frequency of CD8⁺ T cells and (C) numbers per spleen of antigen-specific P14 cells on day 45 post immunization were plotted in the bar graphs. Data shown are representative of at least three independent experiments (* denotes to p<0.05). Note, the baseline number of naïve P14 CD8 T cells without DC-33 immunization would be ~5×10³ cells/ mouse.