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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Immunol. 2014 Mar 21;192(9):4221–4232. doi: 10.4049/jimmunol.1302569

Figure 2. TLR4 ligands LPS and MPLA differentially regulated effector and memory CD8+ T cell differentiation.

Figure 2

B6 mice containing a small number of naive P14 CD8+ T cells were immunized with either DC-33 alone or in combination with LPS or MPLA. (A) Spleens were harvested at days 5, 7 and 45 post immunization, and antigen-specific P14 CD8+ T cells were enumerated and plotted in the line graphs. (B) The frequency of Thy1.1+ P14 cells of CD8+ T cells and expression of KLRG1 and IL-7R were analyzed at days 7 and 45 post immunization. (C) Day 7 effector and day 45 memory P14 CD8+ T cells generated after DC-33 alone (grey shaded) or in combination with LPS (black dashed line) or MPLA (black solid line) were compared for CD62L, CD27 and CXCR3 expression by flow cytometry. (D) Memory P14 CD8+ T cells were stimulated in vitro with GP-33 peptide and cytokine production (IFNγ, TNF and IL-2) was measured by flow cytometry. Bar graphs show the frequency of cells expressing each of the seven possible combinations of IFNγ, TNF and IL-2. (E) Pie graphs show the fraction of the total response comprising cells expressing three cytokines (3+), any two cytokines (2+), or any one cytokine (1+). Data shown are representative of three independent experiments (* denotes to p<0.05).