Figure 3. LPS promoted memory CD8⁺ T cell mediated protective immunity.

(A) B6 mice were immunized with either DC-33 alone or in combination with LPS or MPLA. Eight weeks later, each group of mice was infected with LCMV-Clone13 (2×10⁶ pfu/mouse) and virus-specific CD8⁺ T cells were monitored 4 and 7 days later. Groups of naïve, unimmunized mice were included as controls. (B) Line graphs show the frequency of D^bGP_33-41 tetramer⁺ CD8⁺ T cells in the blood on days 4 and 7 following Clone13 infection. (C) Bar graph shows the number of splenic D^bGP_33-41 tetramer⁺ CD8⁺ T cells at day 7 post infection (p.i.). (D) Viral titers were measured in the serum at days 4 and 7 p.i. and plotted as a line graph. Data shown are representatives of three independent experiments (* denotes to p<0.05).