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Time course of TLRs expressions in Hep-2 cells in response to sH1N1 virus. Hep-2 cells were exposed to sH1N1 virus at a moi 1.0 or 0.01 for 1, 8, 24, 48, 72 or 96 hr and the expression of TLR3 (a) and TLR9 (b) mRNA were then determined by qRT-PCR. Transcript levels normalized to GAPDH and expressed relative to unstimulated cells. Data are the averages±S.D. of triplicate determinations.
