Figure 4. Molecular inhibition of miR-15b is neuroprotective against neuronal ischemia in vitro.
(A,B) Neuroprotection afforded by sevoflurane preconditioning is significantly decreased by transduction of miR-15b (15b) in terms of cell viability (A) and cell death (B). (C,D) Transduction with the miR-15b inhibitor (15bI) effectively promotes cell viability (C) and decreases cell death (D) following OGD. Viability and death were measured by Alamar blue uptake and LDH release. Asterisks indicate comparisons between OGD-only cultures to cultures treated with OGD and transduced with the indicated lentiviral vectors. All data are mean ± SEM, from at least three indepebdent experiments, **P<0.01; and #P<0.05 compared with transduction of either miR-15b (15b) or the inhibitor miR-15B-I (15bI).