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. 2014 Jun 26;4:5452. doi: 10.1038/srep05452

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Foci forming unit reduction assay (FFURA) on Vero cells was performed to evaluate the virucidal activity of baicalin on DENV-2 extracellular particles. Virucidal activity of 50 μg/ml of baicalin (B); or with 25 μg/ml of baicalin (C) or without baicalin (D) using DENV foci immunostaining method on the day 4 post infection were shown. It is shown that 50 μg/ml of baicalin can inactivate more than 99% of DENV-2 free particles (A). Data from triplicate assays for three independent experiments were plotted using Graph Pad Prism Version 5 (Graph Pad Software Inc., San Diego, CA.).