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. 2014 Jun 26;4:5462. doi: 10.1038/srep05462

Table 3. Strains and plasmids for triparental conjugation. Bacteria containing the plasmid pBBRGFP-45 (Kmr, 50 mg·L−1), bacteria containing the plasmid pRK2013 (Kmr, 50 mg·L−1), and strain Ph6 (Cmr, 50 mg·L−1).

Strain or plasmid Genotype or phenotype Source or reference
pBBRGFP-45 pBBR-MCS2 vector with 1.4 kb foreign fragment (gfp) Yu48
pRK2013 mob+, tra+, Kmr, helper plasmid This laboratory
Pseudomonas sp. Ph6 Phenanthrene degradation This laboratory