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. 2014 Jun 26;4:5455. doi: 10.1038/srep05455

Figure 2.

Figure 2

(a) Absorption spectra of the combination of D274A NB-protein and Chl c. Inset: EPR spectra of this combination. (b) Absorption spectra of the combination of WT NB-protein and Chl c. (c) Absorption spectra of the combination of D274A NB-protein and Pchlide. Inset: EPR spectra of this combination. All the absorption spectra were measured at 5 °C at varied times as shown in Figure after initiation of the reaction by adding ATP. EPR spectra were measured at 20 K after rapid freeing of the samples at times indicated in Figures as described in Methods. Note that the time courses are for the reaction at 5 °C and are expected to be significantly slower than those at physiological temperatures.