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. 2014 May 27;124(7):2935–2946. doi: 10.1172/JCI75157

Figure 6. Engagement of β2AR augments PM-induced release of IL-6 from alveolar macrophages in vitro.

Figure 6

(A) We treated primary alveolar macrophages isolated from Adrb2–/– mice (Adrb1+/+Adrb2–/–) and their wild-type littermate controls (Adrb1+/+Adrb2+/+) with PM (10 μg/cm2) or control (medium) and measured IL-6 levels in the medium 24 hours later. (B) We treated murine alveolar macrophages (MH-S cells) with PM (10 μg/cm2) or control (medium) and with albuterol (10–7M) or control (medium) in the presence or absence of propranolol (10 μM) (or control [PBS]) and measured IL-6 levels in the medium 24 hours later. (C) We treated MH-S cells with PM (10 μg/cm2) or control (medium) and with the (S) or (R) enantiomer of albuterol (10–7M) or control. *P < 0.05 for comparison between PM-treated cells with or without albuterol or forskolin; *P < 0.05, PM vs. PBS; **P < 0.05, albuterol vs. control, and simultaneous vs. control; ‡P < 0.05, Adrb1+/+Adrb2–/– vs. Adrb1+/+Adrb2+/+, propranolol vs. control.