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. 2014 May 27;124(7):2935–2946. doi: 10.1172/JCI75157

Figure 7. β2 Agonist therapy and forskolin augment PM-induced release of IL-6 only when administered following exposure to PM.

Figure 7

We treated MH-S cells with PM (10 μg/cm2) or control (medium) and with (A) albuterol (10–7M) or (B) forskolin (50 μM) and control administered simultaneously with the PM or 1 hour after the PM. (C) We treated MH-S cells with PM (10 μg/cm2) or control (medium) and with forskolin (50 μM) or control (saline) 1 hour after PM treatment in the absence of presence of an adenylyl cyclase (AC) inhibitor (SQ22536, 300 μM) (or control [H2O]) and measured IL-6 levels in the medium 24 hours later. (D) We treated MH-S cells with PM (10 μg/cm2) or control (medium) and with forskolin (50 μM) or control 1 hour after PM treatment in the absence or presence of IBMX (1 μM) and aminophylline (10 μM) and measured cAMP levels in cell lysates 1 minute later. *P < 0.05, PM vs. PBS; **P < 0.05, albuterol or forskolin (1 hour after PM) vs. control and albuterol or forskolin (simultaneous with PM ); P < 0.05, AC inhibitor vs. control.