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. 2014 Jun 17;124(7):2963–2976. doi: 10.1172/JCI71630

Figure 6. UPEC bound to A-ICs.

Figure 6

(A) In the C3H/HeN model of pyelonephritis, GFP-labeled UPEC (green) specifically bound A-ICs (marked by apical v-ATPase; red; n = 4). Original magnification, ×40. (B) High-resolution image of UPEC-GFP bound to A-ICs. Original magnification, ×100. (C) UPEC induced Lcn2 (arrowheads) in C3H medullary cells/ICs in kidney (paraffin in situ hybridization). (D) Treatment of an IC line with killed UPEC (24 hours; n = 4) induced Lcn2 message. NF-κB inhibitor (5 μM) reversed the LCN2 signal. (E) Coculture of Lcn2-Luc2 kidney medullary cells with living UPECs (3 hours) induced LCN2-Luc2 reporters. Gentamicin reversed the LCN2 signal (n = 3). Note the luminescent wells (blue, baseline; green/red, activated). Scale bars: 50 μm (A and C); 10 μm (B).