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. 2014 May 27;124(7):2891–2908. doi: 10.1172/JCI70982

Figure 2. MAOA regulates HIF1α stability.

Figure 2

(A) Immunoblots of nuclear extracts from PC-3 (vector and MAOA-overexpression) cells cultured at 21% O2 for HIF1α. (B and D) Immunoblots of paired PC-3 (B) or LNCaP (D) cells cultured at 1% O2 for the indicated time. (C and E) Fold induction of HIF1α target genes in response to hypoxia (1% O2, 24 hours) measured by qPCR in PC-3 (vector and MAOA-overexpression) (C) or LNCaP (shCon and shMAOA) (E) cells. Normoxic gene expression in control PC-3 (vector-expressing) or LNCaP (shCon) cells was used as a control for normalization. Data represent the mean ± SEM (n = 3). *P < 0.05, **P < 0.01.